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human eb1 gfp vector jb131  (Addgene inc)


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    Addgene inc human eb1 gfp vector jb131
    Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the <t>EB1-GFP</t> plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.
    Human Eb1 Gfp Vector Jb131, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human eb1 gfp vector jb131/product/Addgene inc
    Average 94 stars, based on 19 article reviews
    human eb1 gfp vector jb131 - by Bioz Stars, 2026-03
    94/100 stars

    Images

    1) Product Images from "GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells"

    Article Title: GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25063181

    Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.
    Figure Legend Snippet: Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.

    Techniques Used: Transfection, Plasmid Preparation

    Citrullination does not alter polymerization speed but contributes to GnRHa-induced MT lifetime. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and live cell images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Images are kymographs generated in Fiji software version 2.14.0/1.54f showing MT tracks running parallel (or along) the kymograph’s distance axis. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Bar graphs depict the quantitative analysis of track polymerization speeds ( B ) and track lifetimes ( C ) ( n = 30) measured in Fiji software version 2.14.0/1.54f using Rietdorff’s kymograph plugin. Each circle represents an individual observation (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD. Asterisks indicate a significant difference between groups (*** = p < 0.001) and error bars represent the SEM.
    Figure Legend Snippet: Citrullination does not alter polymerization speed but contributes to GnRHa-induced MT lifetime. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and live cell images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Images are kymographs generated in Fiji software version 2.14.0/1.54f showing MT tracks running parallel (or along) the kymograph’s distance axis. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Bar graphs depict the quantitative analysis of track polymerization speeds ( B ) and track lifetimes ( C ) ( n = 30) measured in Fiji software version 2.14.0/1.54f using Rietdorff’s kymograph plugin. Each circle represents an individual observation (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD. Asterisks indicate a significant difference between groups (*** = p < 0.001) and error bars represent the SEM.

    Techniques Used: Transfection, Plasmid Preparation, Generated, Software



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    Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the <t>EB1-GFP</t> plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.
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    Image Search Results


    Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells

    doi: 10.3390/ijms25063181

    Figure Lengend Snippet: Citrullination does not alter the number of MT comets. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Image stacks were used to analyze the number of EB1 comets passing through 10 μm long sections ( n = 3 sections) in each cell ( n = 10 cells; n = 30 sections total); see the representative line overlays in the images. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Kymographs (bottom row) show EB1-GFP comets passing transversely through the lines depicted in the corresponding images of cells (top row), with time on the vertical axis and distance on the horizontal axis. ( B ) The bar graph depicts quantitative analysis of the fold change in the flux of EB1-GFP comets, in comets per second, through the 10 μm sections. Each circle represents an individual comet (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD and error bars represent the SEM.

    Article Snippet: The Human EB1 GFP vector (JB131) was a gift from Tim Mitchison and Jennifer Tirnauer (Addgene plasmid # 39299; http://n2t.net/addgene:39299 (accessed on 29 March 2021); RRID: Addgene_39299).

    Techniques: Transfection, Plasmid Preparation

    Citrullination does not alter polymerization speed but contributes to GnRHa-induced MT lifetime. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and live cell images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Images are kymographs generated in Fiji software version 2.14.0/1.54f showing MT tracks running parallel (or along) the kymograph’s distance axis. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Bar graphs depict the quantitative analysis of track polymerization speeds ( B ) and track lifetimes ( C ) ( n = 30) measured in Fiji software version 2.14.0/1.54f using Rietdorff’s kymograph plugin. Each circle represents an individual observation (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD. Asterisks indicate a significant difference between groups (*** = p < 0.001) and error bars represent the SEM.

    Journal: International Journal of Molecular Sciences

    Article Title: GnRH Induces Citrullination of the Cytoskeleton in Murine Gonadotrope Cells

    doi: 10.3390/ijms25063181

    Figure Lengend Snippet: Citrullination does not alter polymerization speed but contributes to GnRHa-induced MT lifetime. ( A ) LβT2 cells were transfected with the EB1-GFP plasmid for 48 hr, pre-treated with DMSO or 1 μm BB-ClA for 12 h, serum starved, and then stimulated with 10 nM GnRHa for 0 and 30 min. The cells were then placed in a Tokai Hit-STX Stage Top Incubator at 37 °C with 5% CO 2 , and live cell images were captured at 0.5 s intervals for 65–94 s by an SDCM using a 60X oil objective. Images are kymographs generated in Fiji software version 2.14.0/1.54f showing MT tracks running parallel (or along) the kymograph’s distance axis. Line colors indicate different treatment groups (Red = Vehicle, Blue = GnRHa 30, Purple = BB-ClA, Yellow = GnRHa 30 + BB-ClA). Bar graphs depict the quantitative analysis of track polymerization speeds ( B ) and track lifetimes ( C ) ( n = 30) measured in Fiji software version 2.14.0/1.54f using Rietdorff’s kymograph plugin. Each circle represents an individual observation (Red = Vehicle, Blue = GnRHa 30, Green = BB-ClA, Orange = GnRHa 30 + BB-ClA). The difference between the means of each group were separated using Tukey’s HSD. Asterisks indicate a significant difference between groups (*** = p < 0.001) and error bars represent the SEM.

    Article Snippet: The Human EB1 GFP vector (JB131) was a gift from Tim Mitchison and Jennifer Tirnauer (Addgene plasmid # 39299; http://n2t.net/addgene:39299 (accessed on 29 March 2021); RRID: Addgene_39299).

    Techniques: Transfection, Plasmid Preparation, Generated, Software